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Articles

Research

Triple-Blind Randomized Placebo Controlled Research of the Effect of StemEnhance™ on Bone Marrow Stem Cell Mobilization.¹
By: CHRISTIAN DRAPEAU, MSc

1. This is an essence of a full fledge study report got ready for publication in the peer-review scientific literature.
PURPOSE OF THE STUDY
A triple-blinded, randomized, placebo-controlled study on human issues was done on the effect of AFA extracts on the number of circulating stem cells.

METHODS

Consumables
Many volunteers took part in the study and they were fed with one gram of a mixture of two AFA extracts (StemEnhance – SE), and a placebo (Ctrl).

Volunteers
A sum of 15 healthy volunteers were chosen for the study, based upon the following exclusion criteria:

* Bellow 20 or more than more than 65 years of age
* Pregnancy
* Severe asthma and allergies needing medication on daily bases
* Any known chronic disease or previous/current venereal illness
* Repeated recreational drug use
* Damaged digestive function (including any older major gastrointestinal surgery).

When the volunteers arrived, they were seated in quiet and calm areas. They were also instructed to remain quiescent, comfortably sitting in a chair, for one hour. Just after taking the baseline sample, a consumable was given to them. They all were instructed to remain quiescent for the whole period of the trial. Later on, blood samples were taken after 30, 60 and 120 minutes of ingestion of the consumable.

Estimation of circulating stem cells

5 ml blood was taken into heparin and 2 ml blood was taken into EDTA at every time point. One sample was used to do a complete blood count (CBC) and the second sample was made ready for immunostaining (CD34, CD45 and CD14) and flow cytometry. Flow cytometry and the analysis of data were done blindly. Data is shown as mean ± SE.

 

Taking SE became a cause of increase in the number of circulating CD34+ cells, whereas consumption of the placebo did not cause any statistically important effect.

As it is obvious that the overall time for the absorption of bioactive components, delivery to desired organs, and the production a proven physiological response may be different based on overall physiology of every volunteer, we calculated the optimum response of SE at any one point in time. It was done within 60 minutes of consumption.

A 24 ± 5 % increase (median = 27%) in the number of circulating stem cells was recorded with SE. The standard maximum response boosts to 30 ± 6% if we include data taken during a dose study performed in May 2005.

Figure 1. Total time according to the number of CD34+ cells in peripheral blood after taking (arrow) of SE and placebo (Crtl). Data is shown as % of starting value. Bars reveal about standard error.

DISCUSSION

It has been confirmed through this study that SE is efficient in helping the release of stem cells from the bone marrow. It augments the number of circulating stem cells (CD34+ cells). The data collected expresses that SE boosts up the number of circulating stem cells by as much as 30%.

Physiological relevance of the observed stem cell mobilization

The average number of lymphocytes in this study was 1,978 per µL and the standard amount of CD34+ cells at time 0 was 0.12%, for a regular number of circulating CD34+ cells of 2.4 per µL. As seen in this study, an increase of 25% to 30% translates to a normal boost of 0.7 stem cells per µL, for example from 2.4 to 3.1 stem cells per µL. This corresponds to almost 3.5 million new circulating stem cells if 5.0 liters of blood is assumed as sample.

The newness of the idea of using endogenous release of bone marrow stem cells to support maximum health condition goes side by side with a shortage of data linking the magnitude of mobilization with physiological relevance. On the other hand, data exist to guess the physiological relevance of putting into circulation 3.5 million new stem cells. Replying on a number of studies (Orlic et al. 2001; Bodine et al., 1994; Bodine et al. 1993; Vandelverde et al., 2005; Valgimigli et al., 2005) one can calculate roughly that one gram of StemEnhance can lead to potentially of up to a few billion somatic cells in target tissues. For that reason, the standard raise of 25% to 30% found with SE in this study is probably to have physiological relevance.

REFERENCES
Bodine DM, Seidel NE, Gale MS, Nienhuis AW, and. (1994) Efficient Retrovirus Transduction of Mouse Pluripotent Hematopoietic Stem Cells Mobilized Into the Peripheral Blood by Treatment With Granulocyte Colony-Stimulating Factor and Stem Cell Factor. Blood, 84(5): 1482-1491.

Bodine DM, Seidel NE, Zsebo KM, and Orlic D. (1993) In Vivo Administration of Stem Cell Factor to Mice Increases the Absolute Number of Pluripotent Hematopoietic Stem Cells. Blood, 82(2): 445- 455.

Orlic D, Kajstura J, Chimenti S, Bodine DM, Leri A. & Anversa P. (2003) Bone marrow stem cells regenerate infarcted myocardium. Pediatr Transplantation 7 (Suppl. 3): 86–88.

Orlic D, Kajstura J, Chimenti S, Limana F, Jakoniuk I, Quaini F, Nadal-Ginard B, Bodine DM, Leri A. & Anversa P. (2001) Mobilized bone marrow cells repair the infracted heart, improving function and survival. PNAS 98(18):10344–10349.

Valgimigli M, Rigolin GM, Cittanti C, Malagutti P, Curello S, Percoco G, Bugli AM, Porta MD, Bragotti LZ, Ansani L, Mauro E, Lanfranchi A, Giganti M, Feggi L, Castoldi G, Ferrari R. (2005) Use of granulocyte-colony stimulating factor during acute myocardial infarction to enhance bone marrow stem cell mobilization in humans: clinical and angiographic safety profile. Eur Heart J. Apr 28. Epub.
Vandervelde S, van Luyn MJ, Tio RA, Harmsen MC. (2005) Signaling factors in stem cell-mediated repair of infarcted myocardium. J Mol Cell Cardiol. Jun 28. Epub.

ABOUT CHRISTIAN DRAPEAU, MSc
Christian Drapeau MSc, Chief Science Officer of StemTech Health Sciences, Inc. Mr. Drapeau is an author. He is a research scientist and neurophysiologist with more than thirteen years of research experience in natural foods and nutrition industry. Mr.Drapeau has been the Director of Research at Desert Lake Technologies since 2000, engaged with scientific research in association with many universities and research centers on the health advantages of certain botanical raw materials.

Depending on the research he produced and information he studied from other a number of scientific teams, recently Mr. Drapeau and his collaborators together suggested and published a breakthrough idea on adult stem cell physiology. This theory is also the co-inventor of StemEnhance™. Mr. Drapeau got his degree of bachelors in Neurophysiology from McGill University, Montreal, in 1987. He got his degree of masters from the Department of Neurology and Neurosurgery from the Montreal Neurological Institute, McGill University, Montreal, in 1991.

Advance Research

(Gathered from research articles, science papers and medical publications).

Stem Cells From Muscles Can Repair Cartilage

The President's Council on Bioethics: Adult Stem Cells

Stem Cell Therapy in Perspective

Clinical Applications of Blood-Derived and Marrow-Derived Stem Cells for Nonmalignant Diseases . JAMA Vol.299 No. 8, February 27, 2008

Bone marrow stem cells can participate in cutaneous healing, contributing to wound healing - Journal of Cellular Physiology 196, 245-250; 2003

Stem cells participate in the generation of new neurons within the human brain - Proceedings of the National Academy of Sciences USA 100, 1364-1369; 4 Feb 2003

Mobilized stem cells have also been used in renewal of cardiac tissue - Journal of Clinical Investigation 107, 1395-1402; Jun 2001

Bone marrow stem cells have also shown the ability to participate in renewal of retinal tissues - Nature Medicine 8, 1004-1010; Sep 2002